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lambda zap ii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda zap ii vector
    Lambda Zap Ii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%BB+zap+ii+vector/us11932908-1092-12-16
    Average 90 stars, based on 1 article reviews
    lambda zap ii vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Molecular cloning and characterization of a serine proteinase inhibitor from Trichinella spiralis.
    Article Snippet: We produced a recombinant protein from a cDNA library from muscle larvae of Trichinella spiralis which had proteinase inhibitory activity.. The predicted amino acid sequence of the clone had an identity of only 30% to the serine proteinase inhibitors (serpins) from Caenorhabditis elegans or Brugia malayi.. At the putative reactive region, however, the identity was about 50%.

    Article Title: Purified plant expansin proteins and DNA encoding same
    Article Snippet: Poly(A)+RNA was selected by standard procedures (Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor Lab. Press, Plainview, N.Y.), 2nd Ed. (1989)) and used for cDNA synthesis with reverse transcriptase (RT) (Gibco/BRL; type M-MLV). .. A cDNA library was constructed with the λ ZAP II vector, using the Stratagene ZAP cDNA synthesis kit. ..

    Article Title: Identification ofTrichinella spiralisearly antigens at the pre-adult and adult stages
    Article Snippet: Three expression cDNA libraries fromTrichinella spiralisworms 14 h, 20 h and 48 h post-infection (p.i.) were screenedwith serum from pigs experimentally infected with 20000 T. spiralis muscle larvae.. Twenty-nine positive clones were isolated from the 14 h p.i. cDNA library, corresponding to 8 different genes.. A putative excretory-secretory protein similar to that of T. pseudospiralis was identified.

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Article Title: Aberrant DNA methylation as a cancer-inducing mechanism.
    Article Snippet: Ever since methylcytosine was found in genomic DNA, this epigenetic alteration has become a center of scientific attraction, especially because of its relation to gene silencing in disease.. There is currently a wide range of methods designed to yield quantitative and qualitative information on genomic DNA methylation.. The earliest approaches were concentrated on the study of overall levels of methylcytosine, but more recent efforts have focused on the study of the methylation status of specific DNA sequences.

    cDNA Library Assay:

    Article Title: Purified plant expansin proteins and DNA encoding same
    Article Snippet: Poly(A)+RNA was selected by standard procedures (Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor Lab. Press, Plainview, N.Y.), 2nd Ed. (1989)) and used for cDNA synthesis with reverse transcriptase (RT) (Gibco/BRL; type M-MLV). .. A cDNA library was constructed with the λ ZAP II vector, using the Stratagene ZAP cDNA synthesis kit. ..

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. Molecular Cloning of Rat MDC and CCR4 A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Construct:

    Article Title: Purified plant expansin proteins and DNA encoding same
    Article Snippet: Poly(A)+RNA was selected by standard procedures (Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor Lab. Press, Plainview, N.Y.), 2nd Ed. (1989)) and used for cDNA synthesis with reverse transcriptase (RT) (Gibco/BRL; type M-MLV). .. A cDNA library was constructed with the λ ZAP II vector, using the Stratagene ZAP cDNA synthesis kit. ..

    cDNA Synthesis:

    Article Title: Purified plant expansin proteins and DNA encoding same
    Article Snippet: Poly(A)+RNA was selected by standard procedures (Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor Lab. Press, Plainview, N.Y.), 2nd Ed. (1989)) and used for cDNA synthesis with reverse transcriptase (RT) (Gibco/BRL; type M-MLV). .. A cDNA library was constructed with the λ ZAP II vector, using the Stratagene ZAP cDNA synthesis kit. ..

    Article Title: Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray.
    Article Snippet: .. Briefly, double-stranded cDNA was independently synthesized from each mRNA isolated from three stages of anther tissues (RA1, RA2, and RA3) with a λ ZAPII cDNA synthesis kit (Stratagene, La Jolla, CA, USA), and each cDNA was ligated into the Eco RI site of λ ZAP II vector (Stratagene). .. An equal amount of the ligated cDNA derived from each stage was mixed and packaged in vitro using a Gigapack III extract (Stratagene).

    Hybridization:

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. Molecular Cloning of Rat MDC and CCR4 A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Generated:

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. Molecular Cloning of Rat MDC and CCR4 A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. Molecular Cloning of Rat MDC and CCR4 A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Molecular Cloning:

    Article Title: Mononuclear Cell-Infiltrate Inhibition by Blocking Macrophage-Derived Chemokine Results in Attenuation of Developing Crescentic Glomerulonephritis
    Article Snippet: .. Molecular Cloning of Rat MDC and CCR4 A rat spleen cDNA library in λ ZAP II vector (Stratagene, La Jolla, CA) was screened by hybridization using a rat MDC cDNA probe, which was generated by RT-PCR using rat macrophage RNA as a template with the primers designed according to conserved regions between murine and human MDC cDNA (murine cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AA175762","term_id":"1756884","term_text":"AA175762"}} AA175762 , human cDNA GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_002990","term_id":"300360575","term_text":"NM_002990"}} NM_002990 ). .. PCR amplification was carried out using a proofreading DNA polymerase (platinum Pfx DNA polymerase; Invitrogen, Carlsbad, CA).

    Clone Assay:

    Article Title: Aberrant DNA methylation as a cancer-inducing mechanism.
    Article Snippet: Ever since methylcytosine was found in genomic DNA, this epigenetic alteration has become a center of scientific attraction, especially because of its relation to gene silencing in disease.. There is currently a wide range of methods designed to yield quantitative and qualitative information on genomic DNA methylation.. The earliest approaches were concentrated on the study of overall levels of methylcytosine, but more recent efforts have focused on the study of the methylation status of specific DNA sequences.

    Isolation:

    Article Title: Aberrant DNA methylation as a cancer-inducing mechanism.
    Article Snippet: Ever since methylcytosine was found in genomic DNA, this epigenetic alteration has become a center of scientific attraction, especially because of its relation to gene silencing in disease.. There is currently a wide range of methods designed to yield quantitative and qualitative information on genomic DNA methylation.. The earliest approaches were concentrated on the study of overall levels of methylcytosine, but more recent efforts have focused on the study of the methylation status of specific DNA sequences.

    Article Title: Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray.
    Article Snippet: .. Briefly, double-stranded cDNA was independently synthesized from each mRNA isolated from three stages of anther tissues (RA1, RA2, and RA3) with a λ ZAPII cDNA synthesis kit (Stratagene, La Jolla, CA, USA), and each cDNA was ligated into the Eco RI site of λ ZAP II vector (Stratagene). .. An equal amount of the ligated cDNA derived from each stage was mixed and packaged in vitro using a Gigapack III extract (Stratagene).

    Synthesized:

    Article Title: Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray.
    Article Snippet: .. Briefly, double-stranded cDNA was independently synthesized from each mRNA isolated from three stages of anther tissues (RA1, RA2, and RA3) with a λ ZAPII cDNA synthesis kit (Stratagene, La Jolla, CA, USA), and each cDNA was ligated into the Eco RI site of λ ZAP II vector (Stratagene). .. An equal amount of the ligated cDNA derived from each stage was mixed and packaged in vitro using a Gigapack III extract (Stratagene).



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